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α-Glucosidase activity of oleanolic acid and its oxidative metabolites: DFT and Docking studies

[ Vol. 15 , Issue. 14 ]

Author(s):

El Hassane Anouar, Nur Shahidatul Shida Zakaria, Ali Alsalme and Syed Adnan Ali Shah   Pages 1148 - 1158 ( 11 )

Abstract:


A natural pentacyclic triterpenoid oleanolic acid 1 and its biotransformed metabolites 2-3 are potential α-glucosidase inhibitors. To elucidate the inhibitory mechanism of compounds 1, 2 and 3 against α-glucosidase, we calculated (i) their electronic and optical properties using DFT and TD-DFT at the B3LYP/6-31G(d) level in gas and IEF-PCM solvent; and (ii) their binding energies to α-glucosidase via docking study. DFT results showed that the α-glucosidase inhibtion is mainly depend on the polarity parameters of the studied compounds. Docking results revealed that the activity increased with binding energies (i.e. the stability of ligand-receptor complex). The specroscopic data of oleanolic acid 1 and its metabolites 2 and 3 are well predicetd for 13C NMR chemical shifts (R2=99%) and 1H NMR chemical shifts (R2=90%); and for (ii) UV/vis spectra. The assignments and interpretation of NMR chemical shifts and bathochromic shift of λMAX absorption bands are discussed.

Keywords:

Chemical shifts, DFT, Docking, α-Glucosidase, NMR, TD-DFT, Oleanolic acid, UV/visible.

Affiliation:

Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA Campus Puncak Alam, 42300 Bandar Puncak Alam, Selangor D.E, Malaysia.



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